Журнал "Гастроэнтерология" Том 54, №4, 2020
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Apolipoprotein A1 and coefficient of atherogenicity at experimental abdominal sepsis
Авторы: V.O. Kavyn
Ivano-Frankivsk National Medical University, Ivano-Frankivsk, Ukraine
Рубрики: Гастроэнтерология
Разделы: Медицинские форумы
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The purpose of this study was to study content the apolipoprotein 1 and coefficient of atherogenicity at experimental abdominal sepsis.
Materials and methods. It was carried out the research of apolipoprotein 1 and coefficient of atherogenicity at experimental abdominal sepsis in 13 rats with experimental abdominal sepsis. It was used male rats. The weight of rats was 150–199 g. Model of abdominal sepsis in rats was made by a way of introducing 30 % stool suspension intraperitoneally. Through 24 hours after the start of the experiment it was carried out blood examination. The coefficient of atherogenicity was defined as the subtraction α-lipoproteins from cholesterol and division on α-lipoproteins.
Results. It was carried out assessment the general condition of rats (condition of the wool, breathing, weight loss, presence or absence of diarrhea, disorder orientation in space and disorder activity in the rat ant other). On the first day of the experiment the content of apolipoprotein 1 decreased to 0.14 ± 0.04 g/l, which could be caused by an increase in endogenous intoxication. It was knows that of apolipoproteins are a protein part of lipoproteins. One of the important functions of apolipoproteins is specific immunological defense against microorganisms. There are isolated data on the relationship between changes of apolioprotein 1 content and the development of sepsis. After evaluating the coefficient of atherogenicity, we obtained results that showed its reduction. The coefficient of atherogenicity was 0.48, which is due to the growth of both α-lipoproteins and the growth of cholesterol. Cholesterol content was increased to 2.11 ± 0.05 mmol/l in these rats. We found changes in lipid metabolism after 24 hours of the experiment which characterized by a violation of the content of α-lipoproteins. Their content increased and amounted to 1.42 ± 0.04 mmol/l compared with control group. In processing the inflammatory process, α-lipoproteins acquire pro-inflammatory properties and promote the outflow of cholesterol from cells. Compared with the control parameters, the content of β-lipoproteins varied slightly and amounted to 0.23 ± 0.02 mmol/l.
Сonclusions. Lipoprotein metabolism take a part in development of abdominal sepsis, as indicated by an increase in cholesterol, α-lipoproteins. Given the conflicting data and the relevance of the topic, the state of lipid metabolism in abdominal sepsis requires further analysis and study.